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High-Performance Thin Layer Chromatography (HPTLC) Application and Advantages


HPTLC is the improved method of TLC which utilizes the conventional technique of TLC in a more improved way. It is also known as flat-bed or planar chromatography as the stationary phase used is flatbed like surface.

HPTLC works on the same principle of separation by adsorption as conventional TLC. The mobile phase or solvent flows through the capillary action. The analytes move according to their affinities towards the stationary phase (adsorbent). The component with more affinity towards the stationary phase travels slower and the components with less affinity towards the stationary phase travel faster.

Steps Involved in HPTLC:




HPTLC is the most flexible, reliable, and cost-efficient separation technique so becomes one of the most widely used methods for analysis in pharmaceutical industries. This technique is also used in clinical chemistry, forensic chemistry, biochemistry, cosmetology, food and drug analysis, environmental analysis, and other areas.

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